{"_buckets": {"deposit": "ed972cb6-f57c-4b69-adb3-24155a60a82f"}, "_deposit": {"id": "7611", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "7611"}, "status": "published"}, "_oai": {"id": "oai:kitami-it.repo.nii.ac.jp:00007611", "sets": ["87"]}, "author_link": ["38975", "38976", "38977"], "item_1646810750418": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_3_alternative_title_198": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "キチン合成酵素遺伝子プロモーターを利用した新規シイタケ形質転換ベクターの構築"}]}, "item_3_biblio_info_186": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "120", "bibliographicPageStart": "117", "bibliographicVolumeNumber": "111", "bibliographic_titles": [{"bibliographic_title": "Journal of Bioscience and Bioengineering"}]}]}, "item_3_description_184": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation.", "subitem_description_type": "Abstract"}, {"subitem_description": "シイタケ・キチン合成酵素遺伝子プロモーターによってハイグロマイシン耐性遺伝子hphを発現させる発現ユニットを有する、シイタケ用の新規ベクター (pLCHS-hph と pChG-bar)を構築した。ランデムに選択した形質転換体におけるhphの発現をRT-PCR法によって確認した。2種類のベクターはシイタケの形質転換に有用であることが明らかとなった。", "subitem_description_type": "Abstract"}]}, "item_3_publisher_212": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_3_select_195": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sato, Toshitsugu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38975", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okawa, Kumiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38976", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirano, Tatsuya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38977", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-11-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "No265.pdf", "filesize": [{"value": "572.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 572300.0, "url": {"label": "No265.pdf", "url": "https://kitami-it.repo.nii.ac.jp/record/7611/files/No265.pdf"}, "version_id": "0a99104c-59e8-4c40-9a69-a67688c2a5d7"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Lentinula edodes", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Novel vector", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Transformation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chitin synthase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Gene expression", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter", "subitem_title_language": "en"}, {"subitem_title": "キチン合成酵素遺伝子プロモーターを利用した新規シイタケ形質転換ベクターの構築", "subitem_title_language": "ja"}]}, "item_type_id": "3", "owner": "1", "path": ["87"], "permalink_uri": "https://kitami-it.repo.nii.ac.jp/records/7611", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-02-25"}, "publish_date": "2014-02-25", "publish_status": "0", "recid": "7611", "relation": {}, "relation_version_is_last": true, "title": ["Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter"], "weko_shared_id": -1}