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キチン合成酵素遺伝子プロモーターを利用した新規シイタケ形質転換ベクターの構築
https://kitami-it.repo.nii.ac.jp/records/7611
44df4190-4fba-48ab-a077-da3d91abff6b
名前 / ファイル | ライセンス | アクション | |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-02-25 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | キチン合成酵素遺伝子プロモーターを利用した新規シイタケ形質転換ベクターの構築 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lentinula edodes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Novel vector | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transformation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chitin synthase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene expression | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
その他のタイトル | ||||||
その他のタイトル | キチン合成酵素遺伝子プロモーターを利用した新規シイタケ形質転換ベクターの構築 | |||||
著者 |
Sato, Toshitsugu
× Sato, Toshitsugu× Okawa, Kumiko× Hirano, Tatsuya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | シイタケ・キチン合成酵素遺伝子プロモーターによってハイグロマイシン耐性遺伝子hphを発現させる発現ユニットを有する、シイタケ用の新規ベクター (pLCHS-hph と pChG-bar)を構築した。ランデムに選択した形質転換体におけるhphの発現をRT-PCR法によって確認した。2種類のベクターはシイタケの形質転換に有用であることが明らかとなった。 | |||||
書誌情報 |
Journal of Bioscience and Bioengineering 巻 111, 号 2, p. 117-120, 発行日 2011-02 |
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出版者 | ||||||
出版者 | Elsevier | |||||
著者版フラグ | ||||||
値 | author | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |