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Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation.", "subitem_description_type": "Abstract"}, {"subitem_description": "\u30b7\u30a4\u30bf\u30b1\u30fb\u30ad\u30c1\u30f3\u5408\u6210\u9175\u7d20\u907a\u4f1d\u5b50\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306b\u3088\u3063\u3066\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50hph\u3092\u767a\u73fe\u3055\u305b\u308b\u767a\u73fe\u30e6\u30cb\u30c3\u30c8\u3092\u6709\u3059\u308b\u3001\u30b7\u30a4\u30bf\u30b1\u7528\u306e\u65b0\u898f\u30d9\u30af\u30bf\u30fc (pLCHS-hph \u3068 pChG-bar)\u3092\u69cb\u7bc9\u3057\u305f\u3002\u30e9\u30f3\u30c7\u30e0\u306b\u9078\u629e\u3057\u305f\u5f62\u8cea\u8ee2\u63db\u4f53\u306b\u304a\u3051\u308bhph\u306e\u767a\u73fe\u3092RT-PCR\u6cd5\u306b\u3088\u3063\u3066\u78ba\u8a8d\u3057\u305f\u30022\u7a2e\u985e\u306e\u30d9\u30af\u30bf\u30fc\u306f\u30b7\u30a4\u30bf\u30b1\u306e\u5f62\u8cea\u8ee2\u63db\u306b\u6709\u7528\u3067\u3042\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002", "subitem_description_type": "Abstract"}]}, "item_3_publisher_212": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_3_select_195": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_access_right": {"attribute_name": "\u30a2\u30af\u30bb\u30b9\u6a29", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sato, Toshitsugu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38975", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okawa, Kumiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38976", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirano, Tatsuya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "38977", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-11-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "No265.pdf", "filesize": [{"value": "572.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 572300.0, "url": {"label": "No265.pdf", "url": "https://kitami-it.repo.nii.ac.jp/record/7611/files/No265.pdf"}, "version_id": "0a99104c-59e8-4c40-9a69-a67688c2a5d7"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Lentinula edodes", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Novel vector", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Transformation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chitin synthase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Gene expression", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter", "subitem_title_language": "en"}, {"subitem_title": "\u30ad\u30c1\u30f3\u5408\u6210\u9175\u7d20\u907a\u4f1d\u5b50\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u5229\u7528\u3057\u305f\u65b0\u898f\u30b7\u30a4\u30bf\u30b1\u5f62\u8cea\u8ee2\u63db\u30d9\u30af\u30bf\u30fc\u306e\u69cb\u7bc9", "subitem_title_language": "ja"}]}, "item_type_id": "3", "owner": "1", "path": ["1/87"], "permalink_uri": "https://kitami-it.repo.nii.ac.jp/records/7611", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-02-25"}, "publish_date": "2014-02-25", "publish_status": "0", "recid": "7611", "relation": {}, "relation_version_is_last": true, "title": ["Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter"], "weko_shared_id": -1}