@article{oai:kitami-it.repo.nii.ac.jp:00007611,
 author = {Sato, Toshitsugu and Okawa, Kumiko and Hirano, Tatsuya},
 issue = {2},
 journal = {Journal of Bioscience and Bioengineering},
 month = {Feb},
 note = {Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation., シイタケ・キチン合成酵素遺伝子プロモーターによってハイグロマイシン耐性遺伝子hphを発現させる発現ユニットを有する、シイタケ用の新規ベクター (pLCHS-hph と pChG-bar)を構築した。ランデムに選択した形質転換体におけるhphの発現をRT-PCR法によって確認した。2種類のベクターはシイタケの形質転換に有用であることが明らかとなった。},
 pages = {117--120},
 title = {Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter},
 volume = {111},
 year = {2011}
}