{"created":"2021-03-01T06:00:03.843758+00:00","id":7611,"links":{},"metadata":{"_buckets":{"deposit":"e105e73b-9bbc-4e8e-9195-3aaa116014d9"},"_deposit":{"id":"7611","owners":[],"pid":{"revision_id":0,"type":"depid","value":"7611"},"status":"published"},"_oai":{"id":"oai:kitami-it.repo.nii.ac.jp:00007611","sets":["1:87"]},"author_link":["38975","38976","38977"],"control_number":"7611","item_1646810750418":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_3_biblio_info_186":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"120","bibliographicPageStart":"117","bibliographicVolumeNumber":"111","bibliographic_titles":[{"bibliographic_title":"Journal of Bioscience and Bioengineering"}]}]},"item_3_description_184":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation.","subitem_description_type":"Abstract"},{"subitem_description":"シイタケ・キチン合成酵素遺伝子プロモーターによってハイグロマイシン耐性遺伝子hphを発現させる発現ユニットを有する、シイタケ用の新規ベクター (pLCHS-hph と pChG-bar)を構築した。ランデムに選択した形質転換体におけるhphの発現をRT-PCR法によって確認した。2種類のベクターはシイタケの形質転換に有用であることが明らかとなった。","subitem_description_type":"Abstract"}]},"item_3_publisher_212":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_3_select_195":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Toshitsugu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"38975","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okawa, Kumiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"38976","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirano, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"38977","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-22"}],"displaytype":"detail","filename":"No265.pdf","filesize":[{"value":"572.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"No265.pdf","url":"https://kitami-it.repo.nii.ac.jp/record/7611/files/No265.pdf"},"version_id":"c23085c6-15ca-4237-9d09-55db13916cd3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Lentinula edodes","subitem_subject_scheme":"Other"},{"subitem_subject":"Novel vector","subitem_subject_scheme":"Other"},{"subitem_subject":"Transformation","subitem_subject_scheme":"Other"},{"subitem_subject":"Chitin synthase","subitem_subject_scheme":"Other"},{"subitem_subject":"Gene expression","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter","subitem_title_language":"en"},{"subitem_title":"キチン合成酵素遺伝子プロモーターを利用した新規シイタケ形質転換ベクターの構築","subitem_title_language":"ja"}]},"item_type_id":"3","owner":"1","path":["87"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-02-25"},"publish_date":"2014-02-25","publish_status":"0","recid":"7611","relation_version_is_last":true,"title":["Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2024-06-17T02:59:03.604276+00:00"}