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A driving simulator that reproduces the behavior of vehicles traveling on uneven road surfaces was used for subjective evaluation of ride quality. At the same time, the variation of heart rate was measured. From the results, it was found that the test subject\u0027s heart rate increased when driving on the uneven roads, and as the vertical acceleration R.M.S. value increased, the variation of heart rate increased. It was also confirmed that as the variation of heart rate increased, the subjective evaluation of ride quality decreased. Linear regression analyses shows that the subjective evaluation could be described with the variation of heart rate as an explaining variable. 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