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The growth of acetic acid bacteria was inhibited under the following conditions ; initial ethanol conc.\uff1e32(g/1), initial acetic acid conc.\uff1e32(g/1)\uff0cpH\uff1c3.2 and culture temperature \uff1e40\u2103.The optimal temperature range for the bacteria growth was 20?35\u2103. A dense cell culture system using an SCM (shaken ceramic membrane) flask was then examined for efficient acetic acid fermentation. 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