{"_buckets": {"deposit": "388a10a4-c3b3-4130-88a9-2e2cefd5b5eb"}, "_deposit": {"id": "8869", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "8869"}, "status": "published"}, "_oai": {"id": "oai:kitami-it.repo.nii.ac.jp:00008869", "sets": ["1:86"]}, "author_link": ["90331", "90332", "90333", "90334", "90335", "90336", "90337", "90324", "90325", "90326", "90327", "90328", "90253", "90330"], "item_1646810750418": {"attribute_name": "\u51fa\u7248\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_3_alternative_title_198": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u30bf\u30d0\u30b3\u304a\u3088\u3073\u30a4\u30cd\u306e\u61f8\u6fc1\u57f9\u990a\u7d30\u80de\u306b\u304a\u3051\u308b\u6e29\u5ea6\u5909\u5316\u5fdc\u7b54\u6027\u306e\u6025\u6fc0\u306a\u30ab\u30eb\u30b7\u30a6\u30e0\u306e\u53d6\u308a\u8fbc\u307f"}]}, "item_3_biblio_info_186": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "89", "bibliographicPageStart": "83", "bibliographicVolumeNumber": "52", "bibliographic_titles": [{"bibliographic_title": "\u4f4e\u6e29\u751f\u7269\u5de5\u5b66\u4f1a\u8a8c", "bibliographic_titleLang": "ja"}, {"bibliographic_title": "Cryobiology and Cryotechnology", "bibliographic_titleLang": "en"}]}]}, "item_3_description_184": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The temperature is one of the key environmental factors surrounding the living organisms in nature. In plant cells, transient increase in cytosolic Ca^\u003c2+\u003e concentration ([Ca^\u003c2+\u003e]_c) occurs as a response to an acute temperature change. Such a rise in [Ca^\u003c2+\u003e]_c reportedly leads to cold acclimatization. Here, we examined the effect of the temperature- shifts from ambient to the lower or higher temperatures, on induction of [Ca^\u003c2+\u003e]_c increases, using suspension-cultured plant cell lines expressing aequorin, a luminescent [Ca^\u003c2+\u003e]_c-reporter. The cultures used here include three tobacco (Nicotiana tabacum L.) cell lines, namely BY-2, Bel-W3 (O_3-sensitive) and Bel-B (O_3-tolerant), and rice (Oryza sativa L., cv. Nipponbare) AQ7 cells. Generally, rice cells and tobacco cells behaved similarly. The levels of [Ca^\u003c2+\u003e]_c spikes induced by cold shock were much greater than those induced by heat shock in all cell lines tested. Effects of inhibitors suggested that the cold shock-induced [Ca^\u003c2+\u003e]_c spike requires the uptake of extracellular Ca^\u003c2+\u003e via plasma membrane Ca^\u003c2+\u003e channels, while the heat shock response might be due to channel-independent leakages of extracellular and organelle Ca^\u003c2+\u003e into cytoplasm. 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