| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-12-16 |
| タイトル |
|
|
タイトル |
Expression of Root Genes in Arabidopsis Seedlings Grown by Standard and Improved Growing Methods |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Arabidopsis roots |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
improved plant-growing method |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
transcriptome |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
photoreceptors |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
spatial expression |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Qu, Yanli
Liu, Shuai
Bao, Wenlong
Xue, Xian
Ma, Zhengwen
Yokawa, Ken
Baluška, František
Wan, Yinglang
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Roots of Arabidopsis thaliana seedlings grown in the laboratory using the traditional plant-growing culture system (TPG) were covered to maintain them in darkness. This new method is based on a dark chamber and is named the improved plant-growing method (IPG). We measured the light conditions in dark chambers, and found that the highest light intensity was dramatically reduced deeper in the dark chamber. In the bottom and side parts of dark chambers, roots were almost completely shaded. Using the high-throughput RNA sequencing method on the whole RNA extraction from roots, we compared the global gene expression levels in roots of seedlings from these two conditions and identified 141 differently expressed genes (DEGs) between them. According to the KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment, the flavone and flavonol biosynthesis and flavonoid biosynthesis pathways were most affected among all annotated pathways. Surprisingly, no genes of known plant photoreceptors were identified as DEGs by this method. Considering that the light intensity was decreased in the IPG system, we collected four sections (1.5 cm for each) of Arabidopsis roots grown in TPG and IPG conditions, and the spatial-related differential gene expression levels of plant photoreceptors and polar auxin transporters, including CRY1, CRY2, PHYA, PHYB, PHOT1, PHOT2, and UVR8 were analyzed by qRT-PCR. Using these results, we generated a map of the spatial-related expression patterns of these genes under IPG and TPG conditions. The expression levels of light-related genes in roots is highly sensitive to illumination and it provides a background reference for selecting an improved culture method for laboratory-maintained Arabidopsis seedlings. |
| 書誌情報 |
International Journal of Molecular Sciences
en : International Journal of Molecular Sciences
巻 18,
号 5,
p. 951,
発行日 2017
|
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1422-0067 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/ijms18050951 |
| 出版者 |
|
|
出版者 |
MDPI |
| 著者版フラグ |
|
|
言語 |
en |
|
値 |
publisher |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
Int. J. Mol. Sci. 2017, 18(5), 951 (2.8 MB)
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