| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-11-05 |
| タイトル |
|
|
タイトル |
Desorption properties of BSA and LSZ on carbonate ion-modified apatite |
|
言語 |
en |
| タイトル |
|
|
タイトル |
炭酸イオン修飾アパタイト上のBSAとLSZの脱離特性 |
|
言語 |
ja |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Hydroxyapatite |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Carbonate ion |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bovine serum lbumin(BSA) |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Egg white lysozyme(LSZ) |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Adsorption and desorption |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Kanno, Toru
SENDAI, Toru
TADA, Kiyoshi
HORIUCHI, Jun-ichi
AKAZAWA, Toshiyuki
ITABASHI, Koji
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Desorption behavior of two proteins; bovine serum albumin (BSA) and egg white lysozyme (LSZ) was followed on hydroxyapatite (HAp) after 72 h-adsorption in a static system, focusing modification effect of carbonate ion on the HAp surface. Desorbed amounts in a 5 mM - phosphate buffer solution (PBS) showed maxima in 2 h for BSA and 1 h for LSZ. The ratio of the maximal desorbed amount to the initially adsorbed one: the desorption rate was chosen as an index of the binding strength of protein to the surface. For LSZ, the desorption rate decreased as increase of the carbonate content, indicating stronger binding. This was ascribed to the increase of the strongly-adsorption site of LSZ: the P site of the c face because the increase of the carbonate in HAp inhibits crystal growth along the c axis and hence increases the c face. On the other hand, any clear dependency was not found for BSA. However, four samples of the various carbonate contents were divided into two groups; one with the higher desorption rate with nearly full and more than full surface coverages and the other with the lower one of 0.42-0.60, assuming to be side-on (multiple-site) adsorption. This suggested that more numbers of weaker binding sites were involved in BSA adsorption on HAp with the higher desorption rate than the lower one. |
| 書誌情報 |
en : Journal of the Ceramic Society of Japan
巻 117,
号 1370,
p. 1101-1104,
発行日 2009-07
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
1882-0743 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.2109/jcersj2.117.1101 |
| 出版者 |
|
|
出版者 |
日本セラミックス協会 |
| 著者版フラグ |
|
|
言語 |
en |
|
値 |
publisher |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
J. of the Ceramic Soc of Japan,117[10],1101-1104 (523.9 kB)
