{"_buckets": {"deposit": "ad8df6f7-bdb8-4668-afa0-0f3f12fe22a7"}, "_deposit": {"id": "8434", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "8434"}, "status": "published"}, "_oai": {"id": "oai:kitami-it.repo.nii.ac.jp:00008434"}, "item_7_alternative_title_18": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\uff08\u9175\u7d20\u51e6\u7406\u3068\u8cea\u91cf\u5206\u6790\u306b\u3088\u308b\u6f06\u30b9\u30c6\u30e9\u30b7\u30a2\u30cb\u30f3\u3068\u30e9\u30c3\u30ab\u30fc\u30bc\u4e2d\u306eN-\u578b\u7cd6\u9396\u306e\u89e3\u6790\uff09"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-09-04", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_7_date_granted_63": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5", "attribute_value_mlt": [{"subitem_dategranted": "2015-09-04"}]}, "item_7_degree_grantor_61": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u6a5f\u95a2", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "\u5317\u898b\u5de5\u696d\u5927\u5b66"}]}]}, "item_7_degree_name_60": {"attribute_name": "\u5b66\u4f4d\u540d", "attribute_value_mlt": [{"subitem_degreename": "\u535a\u58eb\uff08\u5de5\u5b66\uff09"}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Lacquer sap is water-in-oil emulsion consisted of urushiols, polysaccharides,\nglycoproteins, laccase, and stellacyanin that have been used as natural paint in Asian\ncountries. Laccase and stellacyanin are copper?containing glycoproteins, which are\nheavily glycosylated proteins containing 3 and 15 possible N-glycosylation sites\n(Asn-x-Thr/Ser), respectively. Structural studies on their carbohydrate structures\nhave not been accomplished yet.\nIn present study, we isolated laccase and stellacyanin from lacquer acetone\npowder by continuous ion exchange chromatography using Sephadex CM-50 and\nDEAE A-50. Protein glycosylarion was characterized using enzymatic digestion,\nchemical labeling techniques of released N-linked glycan and subsequent mass\nspectrometric analysis MALDI TOF MS an LC/MS/MS, respectively. We found that\nthree N-glycosylation sites (Asn28, 60 and 102) in lacquer stellacyanin all\nglycosylated with same complex-type N-linked glycan, constituted with\nGlcNAc4Man3Gal2Fuc3Xyl1.\nAlso lacquer laccase glycosylated with complex- and also hybride-type N-linked\nglycans that are common plant-specific structural units of ?-xylose, core ?-fucose and\nLewis epitope (GlcNAc-Gal-Fuc) were observed by MALDI TOF MS. LC/MS/MS\nanalysis of glycopeptides revealed that 13 N-glycosylation sites in laccase were\nglycosylated frequently with complex type N-glycan, GlcNAc4Man3Gal2Fuc3Xyl1,\nand among them Asn5, 233 and 381 were also found to be glycosylated with\nhybride?type N-glycan, mainly GlcNAc2-3Man4-5Fuc1Xyl1. Glycopeptides which carry\npossible N-glycosylation sites at Asn 364 and 519 were not confirmed by LC/MS/MS\nanalysis.", "subitem_description_type": "Abstract"}]}, "item_7_dissertation_number_64": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7", "attribute_value_mlt": [{"subitem_dissertationnumber": "10106\u7532\u7b2c146\u53f7"}]}, "item_7_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.19000/00008428", "subitem_identifier_reg_type": "JaLC"}]}, "item_7_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "ETD"}]}, "item_7_subject_16": {"attribute_name": "\u65e5\u672c\u5341\u9032\u5206\u985e\u6cd5", "attribute_value_mlt": [{"subitem_subject": "490", "subitem_subject_scheme": "NDC"}]}, "item_7_text_66": {"attribute_name": "\u7814\u7a76\u79d1\u30fb\u5c02\u653b\u540d", "attribute_value_mlt": [{"subitem_text_value": "\u533b\u7642\u5de5\u5b66\u5c02\u653b"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "TUMURBAATAR, OYUNJARGAL"}], "nameIdentifiers": [{"nameIdentifier": "44409", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-01-12"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Thesis oyunjargal 20150902.pdf", "filesize": [{"value": "2.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2100000.0, "url": {"label": "Thesis oyunjargal 20150902.pdf", "url": "https://kitami-it.repo.nii.ac.jp/record/8434/files/Thesis oyunjargal 20150902.pdf"}, "version_id": "4cb66b5e-81e7-4b1a-88c3-260776582707"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin and Laccase from Rhus vernicifera", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin and Laccase from Rhus vernicifera"}]}, "item_type_id": "7", "owner": "1", "path": ["2/6"], "permalink_uri": "https://doi.org/10.19000/00008428", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2016-09-03"}, "publish_date": "2016-09-03", "publish_status": "0", "recid": "8434", "relation": {}, "relation_version_is_last": true, "title": ["Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin and Laccase from Rhus vernicifera"], "weko_shared_id": 3}