{"_buckets": {"deposit": "cb539fd4-8d99-4f42-8f5d-9c991cf37fc5"}, "_deposit": {"id": "8399", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "8399"}, "status": "published"}, "_oai": {"id": "oai:kitami-it.repo.nii.ac.jp:00008399", "sets": ["87"]}, "author_link": ["44873", "44177", "44178", "44179", "44180"], "item_1646810750418": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_3_biblio_info_186": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-04", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "1685", "bibliographicPageStart": "1679", "bibliographicVolumeNumber": "8 April 2015", "bibliographic_titles": [{"bibliographic_title": "Biotechnology Letters"}]}]}, "item_3_description_184": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Objective: An electroporation procedure for the species was investigated to develop an efficient transformation method for the basidiomycetous fungus Pseudozyma hubeiensis SY62, a strong biosurfactant-producing host. Results: A plasmid, pUXV1emgfp including green fluorescence protein as a reporter gene, was constructed to determine the transformation and expression of foreign genes. Optimal electroporation conditions achieved 44.8 transformants μg?1 plasmid competency (intact cells) without protoplast treatment. Lithium acetate treatments increased the efficiency to approx. Twice that of control experiments. Almost all transformants demonstrated green fluorescence expressed in the transformant cells. Conclusion: The optimal method, successfully applied to several related species, yields sufficient transformant colonies to engineer the host strain.", "subitem_description_type": "Abstract"}]}, "item_3_full_name_183": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "44873", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90533860", "nameIdentifierScheme": "KAKEN", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000090533860 "}], "names": [{"name": "小西, 正朗", "nameLang": "ja"}]}]}, "item_3_publisher_212": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Springer Verlag"}]}, "item_3_relation_191": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.1007/s10529-015-1837-x", "subitem_relation_type_select": "DOI"}}]}, "item_3_rights_192": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "c 2015 Springer Verlag"}]}, "item_3_select_195": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_3_subject_196": {"attribute_name": "日本十進分類法", "attribute_value_mlt": [{"subitem_subject": "474", "subitem_subject_scheme": "NDC"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Konishi, Masaaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "44177", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshida, Yuta", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "44178", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ikarashi, Mizuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "44179", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Horiuchi, Junichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "44180", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-01-12"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "BILE+Yoshida+2015+revised+with+figure.pdf", "filesize": [{"value": "208.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 208200.0, "url": {"label": "BILE+Yoshida+2015+revised+with+figure.pdf", "url": "https://kitami-it.repo.nii.ac.jp/record/8399/files/BILE+Yoshida+2015+revised+with+figure.pdf"}, "version_id": "deae353f-e02b-4b2c-9ff7-054bcfefc379"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Electroporation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Pseudozyma", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Shuttle vector", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Transformation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ustilago", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Efficient and simple electro-transformation of intact cells for the basidiomycetous fungus Pseudozyma hubeiensis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Efficient and simple electro-transformation of intact cells for the basidiomycetous fungus Pseudozyma hubeiensis", "subitem_title_language": "en"}]}, "item_type_id": "3", "owner": "1", "path": ["87"], "permalink_uri": "https://kitami-it.repo.nii.ac.jp/records/8399", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2015-05-11"}, "publish_date": "2015-05-11", "publish_status": "0", "recid": "8399", "relation": {}, "relation_version_is_last": true, "title": ["Efficient and simple electro-transformation of intact cells for the basidiomycetous fungus Pseudozyma hubeiensis"], "weko_shared_id": -1}