| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2015-05-11 |
| タイトル |
|
|
タイトル |
Efficient and simple electro-transformation of intact cells for the basidiomycetous fungus Pseudozyma hubeiensis |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Electroporation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Pseudozyma |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Shuttle vector |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Transformation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Ustilago |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Konishi, Masaaki
Yoshida, Yuta
Ikarashi, Mizuki
Horiuchi, Junichi
|
| 著者別名 |
|
|
|
識別子Scheme |
WEKO |
|
|
識別子 |
44873 |
|
|
識別子Scheme |
NRID |
|
|
識別子URI |
https://nrid.nii.ac.jp/nrid/1000090533860 |
|
|
識別子 |
1000090533860 |
|
|
姓名 |
小西, 正朗 |
|
|
言語 |
ja |
|
|
姓 |
小西 |
|
|
言語 |
ja |
|
|
名 |
正朗 |
|
|
言語 |
ja |
|
|
|
識別子Scheme |
ISNI |
|
|
|
識別子URI |
https://www.isni.org/isni/ |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Objective: An electroporation procedure for the species was investigated to develop an efficient transformation method for the basidiomycetous fungus Pseudozyma hubeiensis SY62, a strong biosurfactant-producing host. Results: A plasmid, pUXV1emgfp including green fluorescence protein as a reporter gene, was constructed to determine the transformation and expression of foreign genes. Optimal electroporation conditions achieved 44.8 transformants μg?1 plasmid competency (intact cells) without protoplast treatment. Lithium acetate treatments increased the efficiency to approx. Twice that of control experiments. Almost all transformants demonstrated green fluorescence expressed in the transformant cells. Conclusion: The optimal method, successfully applied to several related species, yields sufficient transformant colonies to engineer the host strain. |
| 書誌情報 |
Biotechnology Letters
巻 8 April 2015,
p. 1679-1685,
発行日 2015-04
|
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://doi.org/10.1007/s10529-015-1837-x |
| 権利 |
|
|
権利情報 |
c 2015 Springer Verlag |
| 出版者 |
|
|
出版者 |
Springer Verlag |
| 著者版フラグ |
|
|
言語 |
en |
|
値 |
author |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
BILE+Yoshida+2015+revised+with+figure.pdf (208.2 kB)
