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  1. 学術雑誌掲載済論文
  2. 洋雑誌

Efficient and simple electro-transformation of intact cells for the basidiomycetous fungus Pseudozyma hubeiensis

https://kitami-it.repo.nii.ac.jp/records/8399
https://kitami-it.repo.nii.ac.jp/records/8399
ff3bf0b2-9caf-495b-9e1e-43f63238f4e9
名前 / ファイル ライセンス アクション
BILE+Yoshida+2015+revised+with+figure.pdf BILE+Yoshida+2015+revised+with+figure.pdf (208.2 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2015-05-11
タイトル
言語 en
タイトル Efficient and simple electro-transformation of intact cells for the basidiomycetous fungus Pseudozyma hubeiensis
言語
言語 eng
キーワード
主題Scheme Other
主題 Electroporation
キーワード
主題Scheme Other
主題 Pseudozyma
キーワード
主題Scheme Other
主題 Shuttle vector
キーワード
主題Scheme Other
主題 Transformation
キーワード
主題Scheme Other
主題 Ustilago
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
著者 Konishi, Masaaki

× Konishi, Masaaki

WEKO 44177

en Konishi, Masaaki

Search repository
Yoshida, Yuta

× Yoshida, Yuta

WEKO 44178

en Yoshida, Yuta

Search repository
Ikarashi, Mizuki

× Ikarashi, Mizuki

WEKO 44179

en Ikarashi, Mizuki

Search repository
Horiuchi, Junichi

× Horiuchi, Junichi

WEKO 44180

en Horiuchi, Junichi

Search repository
著者別名
姓名 小西, 正朗
言語 ja
抄録
内容記述タイプ Abstract
内容記述 Objective: An electroporation procedure for the species was investigated to develop an efficient transformation method for the basidiomycetous fungus Pseudozyma hubeiensis SY62, a strong biosurfactant-producing host. Results: A plasmid, pUXV1emgfp including green fluorescence protein as a reporter gene, was constructed to determine the transformation and expression of foreign genes. Optimal electroporation conditions achieved 44.8 transformants μg?1 plasmid competency (intact cells) without protoplast treatment. Lithium acetate treatments increased the efficiency to approx. Twice that of control experiments. Almost all transformants demonstrated green fluorescence expressed in the transformant cells. Conclusion: The optimal method, successfully applied to several related species, yields sufficient transformant colonies to engineer the host strain.
書誌情報 Biotechnology Letters

巻 8 April 2015, p. 1679-1685, 発行日 2015-04
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.1007/s10529-015-1837-x
権利
権利情報 c 2015 Springer Verlag
出版者
出版者 Springer Verlag
著者版フラグ
値 author
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
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