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  1. 学位論文
  2. 博士論文

漆糖鎖とペプチド間の相互作用メカニズムの解明

https://doi.org/10.19000/00007681
https://doi.org/10.19000/00007681
56b26801-b900-4f6d-a088-019c8e4d458d
名前 / ファイル ライセンス アクション
00 00 白 玉廷 学位論文 140824.pdf (1.7 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2014-09-05
タイトル
タイトル Elucidation of interaction mechanism between lacquer polysaccharides and proteins
言語 en
タイトル
タイトル 漆糖鎖とペプチド間の相互作用メカニズムの解明
言語 ja
言語
言語 eng
キーワード
言語 en
主題Scheme Other
主題 lacquer
キーワード
言語 en
主題Scheme Other
主題 polysaccharides
キーワード
言語 en
主題Scheme Other
主題 surface plasmon resonance
キーワード
言語 en
主題Scheme Other
主題 HIV
キーワード
言語 en
主題Scheme Other
主題 oligopeptide
キーワード
言語 en
主題Scheme Other
主題 biosensor
資源タイプ
資源 http://purl.org/coar/resource_type/c_db06
タイプ doctoral thesis
ID登録
ID登録 10.19000/00007681
ID登録タイプ JaLC
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
著者 BAI, Yuting

× BAI, Yuting

WEKO 39478

en BAI, Yuting

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抄録
内容記述タイプ Abstract
内容記述 Lacquer polysaccharides, which exist in the sap of Asian lacquer tree, are highly branched acidic polysaccharides with 1,3-β-galactan backbone and glucouronic acid terminals. Lacquer polysaccharides were reported to be antitumor active and blood coagulation promoting effective, which specific activities might be caused from electrostatic attraction between negative-charged carboxyl groups from the uronic acid terminal of polysaccharides, and positive-charged amino groups from target proteins. By use of surface plasmon resonance (SPR), the interaction dynamics between the polysaccharides and proteins can be quantitatively evaluated.
Lacquer polysaccharides are usually found with two molecular weight fractions, 10 x104 and 3.0 x104Dalton in the proportion of about 25% and 75% respectively. In the previous study, the single fraction of 10x104 was reported to be degraded into 3.0 x104 after the sap was collected. The two pure fractions were first isolated in this study by using
Sephadex size exclusive chromatography. It was found that both of the two fractions show the same structure by 1H and 13C NMR characterization. It was also found that addition of ferric or ferrous ion can lead to a gelation of lacquer polysaccharides with lower molecular weights in aqueous solution, suggesting that the degradation may cause by breakage of metal-carboxyl association linkages. Appearance at 1612 cm-1 due to COO- ion of the IR spectra and alkaline degradation of 3.0 x104 fraction into 1.4x104, which halved the molecular weight, could also be a proof of existence of metal-carboxyl association.
In the dynamic study of proteins-polysaccharides interaction by SPR, poly-L-lysine as an all-positive charged polypeptide model was used to be evaluated the activity of lacquer polysaccharides and their derivatives. It was found that the interaction increased with increasing molecular weight of lacquer polysaccharides. In addition, the sulfated derivatives showed significantly high interactions with dissociation-rate constant of kd = 1.74 x10-4 [1/s], association-rate constant of ka = 4.31x104 [1/Ms] and dissociation constant of KD=4.0x104-9 [M] respectively, while original lacquer polysaccharides gave only kd = 3.54 x10-4
[1/s], ka = 4.99 x102 [1/Ms] and KD = 7.10 x10-7 [M]. These result suggest that the sulfate groups, compared to carboxyl groups give stronger attraction to amino groups, and the interaction should be the reason why sulfated lacquer polysaccharides had potent anti-HIV acctivity.
To evaluate the affection of sulfation to anti-HIV activities, lacquer polysaccharides were sulfated 1, 2 and 3 times respectively, by piperidine-N-sulfate to gives sulfated lacquer polysaccharides with different degree of sulfations. According to the amino acid sequence of V3 loop, C-terminus, and CD4-binding domain of HIV surface glycoprotein gp120 were synthesised and immobilized to the sensor chip separately. The C-terminus gave the highest response that increased with increasing the degree of sulfation.
While the association-rate constant was different from the molecular weights, degree of sulfation, and binding ligands, the dissociation-rate constant of lacquer polysaccharide derivatives was not affective so much and gave rather low values. This implies high stability of the interaction and sulfated polysaccharides were expected for long-term effectives and were possible candidates as an anti-HIV medicine in the future.
言語 en
書誌情報
p. 1, 発行日 2013-09
著者版フラグ
言語 en
値 ETD
学位名
学位名 博士(工学)
学位授与機関
学位授与機関名 北見工業大学
学位授与番号
学位授与番号 10106甲第131号
研究科・専攻名
研究科・専攻名 医療工学専攻
学位授与年月日
学位授与年月日 2013-09-06
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