{"created":"2021-03-01T06:01:04.024298+00:00","id":8740,"links":{},"metadata":{"_buckets":{"deposit":"58821c58-6f01-4b58-b435-002647365476"},"_deposit":{"id":"8740","owners":[],"pid":{"revision_id":0,"type":"depid","value":"8740"},"status":"published"},"_oai":{"id":"oai:kitami-it.repo.nii.ac.jp:00008740","sets":["2:6"]},"author_link":["90189"],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-09","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_63":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-09-08"}]},"item_7_degree_grantor_61":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"北見工業大学"}]}]},"item_7_degree_name_60":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（工学）"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Sulfated polysaccharides have specific antiviral activities, which biological mechanism is assumed to the electrostatic interaction between (+)-charged virus surface glycoproteins and (-)-charged sulfate groups. For the elucidation of the mechanism, several oligopeptides referenced by the sequence of Human Immunodeficiency Virus glycoprotein 120 (HIV gp120) and hemagglutinin (HA) of influenza A and B were synthesized by a peptide synthesizer and the interaction with structurally distinct sulfated polysaccharides such as curdlan sulfate and dextran sulfate was analyzed by SPR.  \nIn this study, three oligosaccharides were synthesized from the sequence of the V3 loop, C-terminus, and CD4 binding domain in the HIV gp120. Oligopeptide A from the V3 loop comprises 20 amino acids with seven positively charged lysine and arginine in the sequence. The basic amino acids were relatively dispersed along the sequence compared with that of oligopeptide B. Likewise, oligopeptide B from the C–terminus comprises seven lysine and arginine, also oligopeptide of Influenza A/Yamagata HA and Influenza A/Brisbane HA comprises 23 amino acids with eight positively charged lysine and arginine in the sequence. Oligopeptide C from the CD4 binding domain and Influenza B /Hong Kong from the HA comprises one lysine and next to the biotin. The biotinylated peptides were synthesized by a microwave assisted solid phase peptide synthesizer using Fmoc protected amino acids. The peptides were purified by RP-HPLC and identified the structure by using MALDI TOF MS. Peptides A and B from HIV gp120 were found to have interacted strongly with dextran and curdlan sulfates, however, the peptide C without positively charged amino acids showed no interaction. These results suggest that the interaction was due to the electrostatic interaction between negatively charged sulfate groups and positively charged amino groups of the peptides. The results of influenza HAs, influenza A (Yamagata and Brisbane) and B (Hong Kong) viruses, are also presented. \nCurdlan and dextran sulfates were found to increase the interaction with increasing the molecular weights and degree of sulfation (DS), which were found to be important factors for the antiviral activity of sulfated polysaccharides. Based on the above, suggesting the antivirus mechanism of sulfated polysaccharides to be the electrostatic interaction of negatively charged sulfated polysaccharides and virus surface glycoprotein at the positively charged amino acid regions.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_dissertation_number_64":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"10106甲第182号"}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.19000/00008715","subitem_identifier_reg_type":"JaLC"}]},"item_7_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_7_text_66":{"attribute_name":"研究科・専攻名","attribute_value_mlt":[{"subitem_text_value":"医療工学専攻"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TUNGALAG, BATTULGA","creatorNameLang":"en"},{"creatorName":"トンガラグ, バトトラグ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"90189","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-09-08"}],"displaytype":"detail","filename":"甲182_TUNGALAG BATTULGA.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"甲182_TUNGALAG BATTULGA","objectType":"fulltext","url":"https://kitami-it.repo.nii.ac.jp/record/8740/files/甲182_TUNGALAG BATTULGA.pdf"},"version_id":"5a400a44-7829-4ed6-864a-82d1a79d52d6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Sulfated polysaccharide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HIV gp120 oligopeptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Influenza virus hemagglutinin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Interaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SPR","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Interaction mechanism between sulfated polysaccharides and HIV oligopeptides analyzed by SPR","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Interaction mechanism between sulfated polysaccharides and HIV oligopeptides analyzed by SPR","subitem_title_language":"en"},{"subitem_title":"SPRによる硫酸化糖鎖とHIVオリゴペプチド間の相互作用メカニズムの解析","subitem_title_language":"ja"}]},"item_type_id":"7","owner":"1","path":["6"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-09-08"},"publish_date":"2020-09-08","publish_status":"0","recid":"8740","relation_version_is_last":true,"title":["Interaction mechanism between sulfated polysaccharides and HIV oligopeptides analyzed by SPR"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-12-13T02:22:15.130825+00:00"}