2026-03-06T00:05:06Z https://kitami-it.repo.nii.ac.jp/oai

oai:kitami-it.repo.nii.ac.jp:00008480 2026-02-17T02:32:04Z 1:86

シイタケ菌床栽培初期における植物細胞壁分解酵素活性の変動と菌床中ラッカーゼの役割 Production of plant cell wall-degrading enzymes in Lentinula edodes and the important role of laccase in early stages of solid-state cultivation NAGAI, Masaru SATO, Toshitsugu Laccase Lentinula edodes Lignin-degrading enzymes Plant cell wall-degrading enzymes Solid-state cultivation We studied the production of plant cell wall-degrading enzymes in Lentinula edodes grown on sawdust medium. A mycelial mat was spotted onto the center of a medium plate, which was then incubated at 23℃ for 31 days. Laccase (EC 1.10.3.2) activity was detected before that of other enzymes. Laccase activity increased along with mycelial growth until the leading edge of the mycelium reached the edge of the plate, and then decreased. Decrease of laccase activity was followed by increased activity of other enzymes. Sawdust treated with purified laccase (Lcc1) was more sensitive to degradation by crude enzymes from L. edodes. These results suggest that laccase plays an important role during early stages of solid-state cultivation of L. edodes. CiNiiからの複製http://ci.nii.ac.jp/naid/110009872486 journal article 日本きのこ学会 2014-10 application/pdf 日本きのこ学会誌 : mushroom science and biotechnology 3 22 114 120 https://kitami-it.repo.nii.ac.jp/record/8480/files/110009872486.pdf https://kitami-it.repo.nii.ac.jp/records/8480 eng c 2014 JAPANESE SOCIETY OF MUSHROOM SCIENCE AND BIOTECHNOLOGY