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Prevention of Copper-Induced Calcium Influx and Cell Death by Prion-Derived Peptide in Suspension-Cultured Tobacco Cells Kagenishi, Tomoko Kagenishi Tomoko Yokawa, Ken Kuse, Masaki Isobe, Minoru Bouteau, Francois Kawano, Tomonori open access c 1946 ? 2014: Verlag der Zeitschrift fur Naturforschung. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. Aequorin Copper Phytotoxicity Prion Protein Impact of copper on the oxidative and calcium signal transductions leading to cell death in plant cells and the effects of the copper-binding peptide derived from the human prion protein (PrP) as a novel plant-protecting agent were assessed using a cell suspension culture of transgenic tobacco (Nicotiana tabacum L., cell line BY-2) expressing the aequorin gene. Copper induces a series of biological and chemical reactions in plant cells including the oxidative burst reflecting the production of reactive oxygen species (ROS), such as hydroxyl radicals, and stimulation of calcium channel opening, allowing a transient increase in cytosolic calcium concentrations. The former was proven by the action of specifiC ROS scavengers blocking the calcium responses and the latter was proven by an increase in aequorin luminescence and its inhibition by specifi c channel blockers. Following these early events completed within 10 min, the development of copper-induced cell death was observed during additional 1 h in a dose-dependent manner. Addition of a synthetic peptide (KTNMKHMA) corresponding to the neurotoxic sequence in human PrP, prior to the addition of copper, effectively blocked both calcium influx and cell death induced by copper. Lastly, a possible mechanism of peptide action and future applications of this peptide in the protection of plant roots from metal toxicity or in favour of phytoremediation processes are discussed. Walter de Gruyter GmbH 2014 eng journal article VoR https://kitami-it.repo.nii.ac.jp/records/8867 https://doi.org/10.1515/znc-2009-5-619 1865-7125 Zeitschrift fur Naturforschung C 64 5-6 411 417 https://kitami-it.repo.nii.ac.jp/record/8867/files/Zeitschrift fur Naturforschung C,64(5-6), P411-417.pdf application/pdf 145 KB 2024-06-24