2024-03-28T14:24:57Z
https://kitami-it.repo.nii.ac.jp/oai
oai:kitami-it.repo.nii.ac.jp:00008788
2022-12-13T02:22:20Z
1:87
Protein release properties on carbonate ion-containing apatite in a flow system
KANNO, Toru
INABA, Morito
SENDAI, Toru
HORIUCHI, Jun-ichi
Hydroxyapatie
Carbonate ion
Protein
Adsorption and release
Flow system
Release properties of bovine serum albumin (BSA) and egg white lysozyme (LSZ) on hydroxyapatite containing carbonate ion (CHAp) of various contents were examined with our new flow experimental system. The desorption ratio: the rde, defined as the ratio of desorbed amount to initially-adsorbed one, was employed as an index of binding strength of these proteins to the surface. Dependency of the content of the carbonate ion on the rde showed reverse tendency for BSA and LSZ; the rde of BSA increased as an increase of content of the carbonate ion, while that of LSZ decreased as the increase of the content. This result clarified a difference of primary adsorption sites between BSA and LSZ. As incorporation of the carbonate ion into apatite retarded the growth of the crystal along the c axis, the exposed area of the c face, i.e. the primary adsorption site of LSZ, the p site increased and that of the a (b) face, i.e. the primary site of BSA, the c site decreased.
日本セラミックス協会
2013-12
eng
journal article
VoR
https://kitami-it.repo.nii.ac.jp/records/8788
https://doi.org/10.2109/jcersj2.121.393
1882-0743
Journal of the Ceramic Society of Japan
121
1412
393
396
https://kitami-it.repo.nii.ac.jp/record/8788/files/J. of the Ceramic Soc of Japan,121[4],393-396.pdf
application/pdf
1.4 MB
2019-11-05