2024-03-28T12:58:29Z
https://kitami-it.repo.nii.ac.jp/oai
oai:kitami-it.repo.nii.ac.jp:00008480
2022-12-13T02:22:02Z
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Production of plant cell wall-degrading enzymes in Lentinula edodes and the important role of laccase in early stages of solid-state cultivation
シイタケ菌床栽培初期における植物細胞壁分解酵素活性の変動と菌床中ラッカーゼの役割
シイタケ菌床栽培初期における植物細胞壁分解酵素活性の変動と菌床中ラッカーゼの役割
NAGAI, Masaru
SATO, Toshitsugu
c 2014 JAPANESE SOCIETY OF MUSHROOM SCIENCE AND BIOTECHNOLOGY
Laccase
Lentinula edodes
Lignin-degrading enzymes
Plant cell wall-degrading enzymes
Solid-state cultivation
We studied the production of plant cell wall-degrading enzymes in Lentinula edodes grown on sawdust medium. A mycelial mat was spotted onto the center of a medium plate, which was then incubated at 23℃ for 31 days. Laccase (EC 1.10.3.2) activity was detected before that of other enzymes. Laccase activity increased along with mycelial growth until the leading edge of the mycelium reached the edge of the plate, and then decreased. Decrease of laccase activity was followed by increased activity of other enzymes. Sawdust treated with purified laccase (Lcc1) was more sensitive to degradation by crude enzymes from L. edodes. These results suggest that laccase plays an important role during early stages of solid-state cultivation of L. edodes.
CiNiiからの複製http://ci.nii.ac.jp/naid/110009872486
日本きのこ学会
2014-10
eng
journal article
VoR
https://kitami-it.repo.nii.ac.jp/records/8480
日本きのこ学会誌 : mushroom science and biotechnology
22
3
114
120
https://kitami-it.repo.nii.ac.jp/record/8480/files/110009872486.pdf
application/pdf
6.7 MB
2017-01-12